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KMID : 1161420210240060660
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Journal of Medicinal Food
2021 Volume.24 No. 6 p.660 ~ p.665
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Evaluation In Vitro of Toxicity of Hydroalcoholic Extract of Leaves and Roots from Yacon (Smallanthus sonchifolius)
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Martinez-Oliveira Patricia
Zuravski Luisa
de Oliveira Micaela Federizzi
Stroher Deise Jaqueline
Coelho Ritiele Pinto
Pilar Bruna Cocco
de Oliveira Luis Flavio Souza
da Costa Escobar Piccoli Jacqueline
Machado Michel Mansur
Denardin Cristiane Casagrande
Manfredini Vanusa
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Abstract
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Yacon is an Andean plant that has been used in folk medicine for its medicinal properties. The beneficial effects of this plant are possibly due to the high content of phenolic compounds present in its leaves and roots. This study evaluated the in vitro toxicity of the hydroalcoholic extract of leaves and roots from yacon (1, 10, 50, and 100?μg/mL) through cell viability tests, genotoxic and mutagenic activity in leukocytes culture cells; and cytotoxicity and apoptosis cell death (1, 10, 50, 100, and 500?μg/mL) in cell line originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol, so-called 3T3 protocol “3-day transfer, inoculum 3?×?105 cells” (3T3 cell line). No mutagenic and cytotoxic activities were observed in leukocyte cultures. Cytotoxic activity was evidenced in the highest concentrations of yacon leaf extract (50 and 100?μg/mL), whereas all concentrations tested with yacon leaf extract there was induction for apoptosis in the 3T3 cells. Genotoxic potential was observed only at higher doses of leaf (50 and 100?μg/mL) and root (100?μg/mL) extract. These results suggest that yacon leaf at high concentrations may present toxic potential showing concentration-dependent behavior; however, in vivo studies should be performed to validate these results.
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KEYWORD
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apoptosis, cytotoxicity, genotoxic activity, mutagenic activity, yacon
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